Regional brain variations of cytochrome oxidase staining during olfactory learning in the honeybee (Apis mellifera).

Patrice Déglise (1) , Matthieu Dacher (1) , Emmanuelle Dion (1) , Monique Gauthier (1) , Catherine Armengaud (1)

1 CRCA - Centre de Recherches sur la Cognition Animale - UMR5169

Regional brain variations of cytochrome oxidase (CO) staining were analyzed in the honeybee (Apis mellifera) after olfactory conditioning of the proboscis extension reflex. Identification of brain sites where stimuli converge was done by precise image analysis performed in antennal lobes (AL) and mushroom bodies (MB). In Experiment 1, bees received 5 odorant stimulations that induced a transient decrease of CO activity in the lateral part of the AL. In Experiment 2, bees were trained with 5-trial olfactory conditioning. CO activity transiently increased in the lips of the MB calyces. There was also a delayed increase in the lateral part of the AL. An olfactory stimulus presented alone and an odor paired to a sucrose stimulation are treated by different pathways, including both AL and MB.

Domaines: Neurosciences

Patrice Déglise, Matthieu Dacher, Emmanuelle Dion, Monique Gauthier, Catherine Armengaud. Regional brain variations of cytochrome oxidase staining during olfactory learning in the honeybee (Apis mellifera).. Behavioral Neuroscience, 2003, 117 (3), pp.540-7. ⟨10.1037/0735-7044.117.3.540⟩. ⟨hal-00319303⟩