Numerical investigation of subgrid mixing effects on the calculation of biological reaction rates

Marion Linkes (1, 2) , Pascal Fede (1, 2) , Jérôme Morchain (3) , Philippe Schmitz (3)

1 FERMAT - Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts
2 IMFT - Institut de mécanique des fluides de Toulouse
3 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés

The consequences of substrate concentration heterogeneities at the cell level, on the behavior of microbial populations have been identified some years ago. However, subgrid effects are rarely considered in bioreactor modelling. In this paper, this central issue is investigated with Direct Numerical Simulations (DNS) coupled with Lagrangian particle tracking and scalar field calculations in the case of statistically steady homogeneous and isotropic turbulence. From these calculations, the exact distribution of substrate uptake rates of a microorganism population is calculated and compared, favorably, to analytical solutions. A metabolic model considering anabolism, oxidative catabolism and dissimilation is invoked to quantify the consequences in terms of overall reaction rates at the population scale. It is shown that imperfect mixing reduces the growth rate and increases the by-product formation while leaving the total uptake rate unchanged. This work provides a rational explanation, based on physical consideration, for the loss in biomass productivity and the increase of by-product formation in imperfectly mixed bioreactors.

Mots clés: Bioreactors; Mixing; Metabolic model; Direct numerical simulation; Statistical approach; dissolved-oxygen concentration; escherichia-coli; glucose; fermentations; populations; metabolism; turbulence; batch; simulation; large-scale bioreactors

Domaines: Biotechnologies

Marion Linkes, Pascal Fede, Jérôme Morchain, Philippe Schmitz. Numerical investigation of subgrid mixing effects on the calculation of biological reaction rates. Chemical Engineering Science, 2014, 116, pp.473 - 485. ⟨10.1016/j.ces.2014.05.005⟩. ⟨hal-01268895⟩