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Poster communications

Benefits of PacBio HiFi long reads for metagenomic whole genome analysis

Abstract : Whole metagenomics analysis aim to provide an overview of the biodiversity and the functions of bacterial communities. Currently, those analysis are performed on Illumina short read sequencers that provide the advantage of generating less than 1% sequencing error. The arrival of the HiFi technology (PacBio Sequel II) allow us to sequence long read fragments of several Kb with a low error rate. One of the aim of the SeqOccIn project (Sequencing Occitanie Innovation, https://get.genotoul.fr/seqoccin/) is to acquire expertise in metagenomics to better characterize microbial communities inside complex matrices. In the framework of this project, we developed the metagenomics shotgun protocol on the PacBio Sequel II sequencer and carry out a comparative study of the assemblies obtained with PacBio long reads and Illumina short reads data.
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Poster communications
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https://hal.archives-ouvertes.fr/hal-03538657
Contributor : Jean Mainguy Connect in order to contact the contributor
Submitted on : Friday, January 21, 2022 - 10:55:14 AM
Last modification on : Monday, May 23, 2022 - 12:04:03 PM
Long-term archiving on: : Friday, April 22, 2022 - 7:03:04 PM

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  • HAL Id : hal-03538657, version 1

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Adrien Castinel, Jean Mainguy, Olivier Bouchez, Sylvie Combes, Carole Iampietro, et al.. Benefits of PacBio HiFi long reads for metagenomic whole genome analysis. Environmental And Agronomical Genomics Symposium, Oct 2021, Tours, France. ⟨hal-03538657⟩

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