Creation of Three-dimensional Liver Tissue Models from Experimental Images for Systems Medicine

Stefan Hoehme (1) , Adrian Friebel (1) , Seddik Hammad (2, 3) , Dirk Drasdo (4) , Jan G. Hengstler (3)

1 IZBI - Interdisciplinary Centre for Bioinformatics [Leipzig]
2 Department of Forensic Medicine and Veterinary Toxicology [Qena]
3 IFADO - Leibniz Research Centre for Working Environment and Human Factors [Dortmund]
4 MAMBA - Modelling and Analysis for Medical and Biological Applications

In this chapter, we illustrate how three-dimensional liver tissue models can be created from experimental image modalities by utilizing a well-established processing chain of experiments, microscopic imaging, image processing, image analysis and model construction. We describe how key features of liver tissue architecture are quantified and translated into model parameterizations, and show how a systematic iteration of experiments and model simulations often leads to a better understanding of biological phenomena in systems biology and systems medicine.

Mots clés: Systems medicine; liver tissue model; hepatocyte transplantation; 2D/3D microscopy; liver architecture; confocal scanning microscopy; TiQuant software

Domaines: Modélisation et simulation; Bio-Informatique, Biologie Systémique [q-bio.QM]; Anatomie, Histologie, Anatomopathologie [q-bio.TO]; Hépatologie et Gastroentérologie; Imagerie médicale; Traitement des images [eess.IV]

Stefan Hoehme, Adrian Friebel, Seddik Hammad, Dirk Drasdo, Jan G. Hengstler. Creation of Three-dimensional Liver Tissue Models from Experimental Images for Systems Medicine. Peggy Stock, Bruno Christ. Hepatocyte Transplantation, 1506, Springer, pp.319-362, 2017, Methods in Molecular Biology, 978-1-4939-6504-5 (Print) 978-1-4939-6506-9. ⟨10.1007/978-1-4939-6506-9_22⟩. ⟨hal-01426722⟩