[Evaluation of the Rapid-ATB system for testing the sensitivity of staphylococci to antibiotics. Comparison with the agar dilution reference method].

P Le Noc , G. Carret (1) , C. Couix (2) , Jean-Pierre Flandrois (3, 1) , J Gayral , J. Robert

1 LBBE - Laboratoire de Biométrie et Biologie Evolutive - UMR 5558
2 CHLS - Centre Hospitalier Lyon Sud [CHU - HCL]
3 BPGE - Bioinformatique, phylogénie et génomique évolutive

Rapid ATB Staph is a new, automated, four-hour procedure for testing the susceptibility to antibiotics of staphylococci. Results obtained with this method were compared to those recorded using agar dilution. For all tested antibiotics as a group (5 on 201 strains, 8 on 100 strains), overall agreement between the two sets of results was 96%. 1,809 susceptibility tests were performed, with only 24 minor discrepancies (1.3%) mainly involving cotrimoxazole, and 50 major discrepancies (2.7%) mainly involving doxycycline and chloramphenicol. Rapid ATB Staph clearly demonstrates the heterogeneous oxacillin-resistance of staphylococci as well as their inducible resistance to erythromycin. Our results show that this new system is a very accurate means for testing the susceptibility to antibiotics of staphylococci.

Domaines: Bio-Informatique, Biologie Systémique [q-bio.QM]; Evolution [q-bio.PE]; Systématique, phylogénie et taxonomie; Ecosystèmes; Bactériologie

P Le Noc, G. Carret, C. Couix, Jean-Pierre Flandrois, J Gayral, et al.. [Evaluation of the Rapid-ATB system for testing the sensitivity of staphylococci to antibiotics. Comparison with the agar dilution reference method].. Pathologie Biologie, 1986, 34 (5 Pt 2), pp.600-3. ⟨hal-02332697⟩