Simulation of Astrocytic Calcium Dynamics in Lattice Light Sheet Microscopy Images

Anaïs Badoual (1, 2) , Misa Arizono (3) , Audrey Denizot (4) , Mathieu Ducros (5) , Hugues Berry (6, 7) , U Valentin Nägerl (3) , Charles Kervrann (1, 2)

1 SERPICO - Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes
2 Biologie Cellulaire et Cancer
3 IINS - Interdisciplinary Institute for Neuroscience [Bordeaux]
4 OIST - Okinawa Institute of Science and Technology Graduate University
5 BIC - Bordeaux Imaging Center
6 BEAGLE - Artificial Evolution and Computational Biology
7 LIRIS - Laboratoire d'InfoRmatique en Image et Systèmes d'information

Astrocytes regulate neuronal information processing through a variety of spatio-temporal calcium signals. Recent advances in calcium imaging have started to shine light on astrocytic activity, but the complexity and size of the recorded data strongly call for more advanced computational analysis tools. Their development is currently hindered by the lack of reliable, labeled annotations that are essential for the evaluation of algorithms and the training of learning-based methods. To solve this labeling problem, we have designed a generator of 2D/3D lattice light sheet microscopy (LLSM) sequences which realistically depict the calcium dynamics of astrocytes. By closely modeling calcium kinetics in real astrocytic ramifications, the generated datasets open the door for the deployment of convolutional neural networks in LLSM.

Mots clés: Astrocytes; calcium signals; image analysis; lattice light sheet microscopy; synthetic training datasets

Domaines: Traitement du signal et de l'image [eess.SP]; Modélisation et simulation

Anaïs Badoual, Misa Arizono, Audrey Denizot, Mathieu Ducros, Hugues Berry, et al.. Simulation of Astrocytic Calcium Dynamics in Lattice Light Sheet Microscopy Images. ISBI 2021 - IEEE International Symposium on Biomedical Imaging, Apr 2021, Nice, France. pp.1-5, ⟨10.1109/ISBI48211.2021.9433984⟩. ⟨hal-03106797⟩