Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes.

Arnaud Delaherche (1, 2) , Elisabeth Bon (3) , Aurelien Dupé (1, 2) , Megumi Lucas (4, 5) , Benoit Arveiler (6) , Antoine de Daruvar (3) , Aline Lonvaud-Funel (1, 2)

1 LBMA - Laboratoire de Biotechnologie et Microbiologie Appliquée
2 ISVV - Institut des Sciences de la Vigne et du Vin
3 MABIOVIS - Modèles et algorithmes pour la Bioinformatique et la Visualisation d'informations;
4 VIRTUAL PLANTS - Modeling plant morphogenesis at different scales, from genes to phenotype
5 RHIZOGéNèSE- UMR DIA-PC - Equipe Rhizogenèse
6 Laboratoire de Génétique Humaine, Développement et Cancer

Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown. The study was based on the comparative genomic analysis by subtractive hybridization between two strains of O. oeni with opposite enological potential. The genomic sequences obtained from subtractive hybridization were amplified by polymerase chain reaction and sequenced for the 16 strains. A considerable diversity among strains of O. oeni was observed.

Mots clés: GENES; VARIABILITY

Mots clés: OENOCOCCUS OENI; OENOCOCCUS; OENOCOCCUS OENI SPECIES; BIOLOGIE MOLECULAIRE

Domaines: Bio-Informatique, Biologie Systémique [q-bio.QM]; Bio-informatique [q-bio.QM]; Evolution [q-bio.PE]; Bactériologie

Arnaud Delaherche, Elisabeth Bon, Aurelien Dupé, Megumi Lucas, Benoit Arveiler, et al.. Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes.. Applied Microbiology and Biotechnology, 2006, 73 (2), pp.394-403. ⟨10.1007/s00253-006-0487-2⟩. ⟨inria-00339828⟩