Lipo-chitooligosaccharides as regulatory signals of fungal growth and development

Tomás Allen Rush (1, 2) , Virginie Puech Pagès (3, 4) , Adeline Bascaules (5, 6) , Patricia Jargeat (7) , Fabienne Maillet (8) , Alexandra Haouy (9) , Arthur Quymanh Maes (9) , Cristobal Carrera Carriel (1, 2) , Devanshi Khokhani (1, 2) , Michelle Keller-Pearson (1, 2) , Joanna Tannous (1, 2) , Kevin Cope (1, 2, 10) , Kevin Garcia (1, 2, 11) , Junko Maeda (1, 2) , Chad Johnson (1) , Bailey Kleven (1, 2) , Quanita Choudhury (2, 12, 13) , Jessy Labbe (2) , Candice Swift (14) , Michelle O’malley (14) , Jin Woo Bok (1) , Sylvain Cottaz (15) , Sébastien Fort (15) , Verena Poinsot (16) , Michael Sussman (1) , Corinne Lefort (3) , Jeniel Nett (1) , Nancy Keller (1) , Guillaume Bécard (17) , Jean-Michel Ané (1)

1 University of Wisconsin-Madison
2 ORNL - Oak Ridge National Laboratory [Oak Ridge]
3 Evolution des Interactions Plantes-Microorganismes
4 MetaToul Agromix
5 IMRCP - Interactions moléculaires et réactivité chimique et photochimique
6 IMRCP - BIBAC - IMRCP - Chimie analytique et interactions biomolécules - matière molle biomimétique
7 EDB - Evolution et Diversité Biologique
8 LIPME - Laboratoire des Interactions Plantes Microbes Environnement
9 LRSV - Laboratoire de Recherche en Sciences Végétales
10 SDSTATE - South Dakota State University
11 NC State - North Carolina State University [Raleigh]
12 The University of Tennessee [Knoxville]
13 University of Georgia [USA]
14 UC Santa Barbara - University of California [Santa Barbara]
15 CERMAV - Centre de Recherches sur les Macromolécules Végétales
16 UT3 - Université Toulouse III - Paul Sabatier
17 Interactions Microbiennes dans la Rhizosphère et les Racines

Lipo-chitooligosaccharides (LCOs) are signaling molecules produced by rhizobial bacteria that trigger the nodulation process in legumes, and by some fungi that also establish symbiotic relationships with plants, notably the arbuscular and ecto mycorrhizal fungi. Here, we show that many other fungi also produce LCOs. We tested 59 species representing most fungal phyla, and found that 53 species produce LCOs that can be detected by functional assays and/or by mass spectroscopy. LCO treatment affects spore germination, branching of hyphae, pseudohyphal growth, and transcription in non-symbiotic fungi from the Ascomycete and Basidiomycete phyla. Our findings suggest that LCO production is common among fungi, and LCOs may function as signals regulating fungal growth and development.

Domaines: Mycologie; Biochimie [q-bio.BM]

Tomás Allen Rush, Virginie Puech Pagès, Adeline Bascaules, Patricia Jargeat, Fabienne Maillet, et al.. Lipo-chitooligosaccharides as regulatory signals of fungal growth and development. Nature Communications, 2020, 11, pp.3897. ⟨10.1038/s41467-020-17615-5⟩. ⟨hal-02916284⟩