Paneth cell defects induce microbiota dysbiosis In mice and promote visceral hypersensitivity
Résumé
Separation of newborn rats from their mothers induces visceral hypersensitivity and impaired epithelial secretory cell lineages when they are adults. Little is known about the mechanisms by which maternal separation causes visceral hypersensitivity or its relationship with defects in epithelial secretory cell lineages. We performed studies with C3H/HeN mice separated from their mothers as newborns and mice genetically engineered (Sox9flox/flox-vil-cre on C57BL/6 background) to have deficiencies in Paneth cells. Paneth cells deficiency was assessed by lysozyme staining of ileum tissues and lysozyme activity in fecal samples. When mice were 50 days old, their abdominal response to colorectal distension was assessed by electromyography. Fecal samples were collected and microbiota were analyzed using GULDA quantitative PCR. Mice with maternal separation developed visceral hypersensitivity and defects in Paneth cells, as reported from rats, compared to mice without maternal separation. Sox9flox/flox-vil-Cre mice also had increased visceral hypersensitivity compared to control littermate Sox9flox/flox mice. Fecal samples from mice with maternal separation and from Sox9flox/flox-vil-cre mice had evidence for intestinal dysbiosis of the microbiota, characterized by expansion of Escherichia coli. Daily gavage of conventional C3H/HeN adult mice with 109 commensal E. coli induced visceral hypersensitivity. Conversely, daily oral administration of lysozyme prevented expansion of E. coli during maternal separation and visceral hypersensitivity. Mice with defects in Paneth cells (induced by maternal separation or genetically engineered) have intestinal expansion of E. coli leading to visceral hypersensitivity. These findings provide evidence that Paneth cell function and intestinal dysbiosis are involved in visceral sensitivity.
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers produits par l'(les) auteur(s)
Loading...