1H NMR and MVA metabolomic profiles of urines from piglets fed with boluses contaminated with a mixture of five mycotoxins
Résumé
Metabolic profile of urine from piglets administered with single boluses contaminated with mycotoxin mixture
(deoxynivalenol, aflatoxin B1, fumonisin B1, zearalenone, and ochratoxin A) were studied by 1H NMR
spectroscopy and chemometrics (PCA, PLS-DA, and OPLS-DA). The mycotoxin levels were close to the
established maximum and guidance levels for animal feed (2003/100/EC and 2006/576/EC). Urine samples
were obtained from four groups of four piglets before (control, C) or within 24 h (treated, T) after receiving a
contaminated boluses with increasing doses of mycotoxins (boluses 1–4). For the two highest dose groups, the
urines were collected also after one week of wash out (W). For the two lowest doses groups no significant
differences between the C and T samples were observed. By contrast, for the two highest doses groups the T
urines separated from the controls for a higher relative content of creatinine, p-cresol glucuronide and phenyl
acetyl glycine and lower concentration of betaine and TMAO. Interestingly, a similar profile was found for both
W and T urines suggesting, at least for the highest doses used, serious alteration after a single bolus of mycotoxin
mixture.
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