Determination of fumonisin B1 levels in body fluids and hair from piglets fed fumonisin B -contaminated diets

Pollyana C.M.C. Souto (1) , Alessandra V. Jager (2) , Fernando G. Tonin (3) , Tânia Petta (1) , Mayra C. Di Gregório (1) , Anne Marie Cossalter (4) , Philippe Pinton (5) , Isabelle P. Oswald (5) , George E. Rottinghaus (6) , Carlos A.F. Oliveira (1)

1 Department of Food Engineering, School of Animal Science and Food Engineering
2 Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirao Preto
3 Department of Biosystems Engineering, School of Animal Science and Food Engineering
4 Ezop - Plateforme Ezop
5 ToxAlim-BioToMyc - Biosynthèse & Toxicité des Mycotoxines
6 Veterinary Medical Diagnostic Laboratory

The levels of fumonisin B1 (FB1) residues in plasma, urine, feces and hair from 24 piglets fed FB1-contaminated diets containing 3.1, 6.1 or 9.0 μg FB1.g-1 for 28 days were determined using liquid chromatography coupled to mass spectrometry (LC-MS/MS). The levels of FB1 in plasma, urine, feces and pooled hair (n = 3) samples varied from 0.15 to 1.08 μg.L-1, 16.09-75.01 μg.L-1, 1.87-13.89 μg.g-1 and 2.08-8.09 ng.g-1, respectively. Significant correlations (r = 0.808-0.885; P < 0.001; N = 18) were found between FB1 intake and plasma FB1 on days 7, 14, 21 and 28. However, urinary FB1 correlated with FB1 intake only on days 7 and 14 (r = 0.561-572; P = 0.02; N = 18). A significant correlation (r = 0.509; P = 0.02; N = 24) was also found for the first time between FB1 in hair samples and FB1 intake. Plasma and urinary FB1 are good biomarkers of early exposure of pigs to low dietary FB1 levels, although plasma is recommended to assess prolonged exposure (>14 days). The possibility to evaluate hair as a biomarker of fumonisin exposure was established, although further studies are needed to provide physiologically based toxicokinetics of residual FB1 in the pig hair.

Mots clés: urine; plasma; exposure assessment; FB(1); hair; biomarker

Domaines: Sciences du Vivant [q-bio]

Pollyana C.M.C. Souto, Alessandra V. Jager, Fernando G. Tonin, Tânia Petta, Mayra C. Di Gregório, et al.. Determination of fumonisin B1 levels in body fluids and hair from piglets fed fumonisin B -contaminated diets. Food and Chemical Toxicology, 2017, 108, pp.1-9. ⟨10.1016/j.fct.2017.07.036⟩. ⟨hal-01605869⟩