Maternal exposure to low levels of corticosterone during lactation protects against experimental inflammatory colitis-induced damage in adult rat offspring

Carla Petrella (1) , Chiara Giuli (1) , Simona Agostini (2) , Valérie Alquier-Bacquié (3) , Manuela Zinni (1) , Vassilia Theodorou (4) , Maria Broccardo (1) , Paola Casolini (1) , Giovanna Improta (1)

1 UNIROMA - Università degli Studi di Roma "La Sapienza" = Sapienza University [Rome]
2 ToxAlim - ToxAlim
3 ToxAlim-TIM - Toxicologie Intégrative & Métabolisme
4 ToxAlim-NGN - Neuro-Gastroentérologie & Nutrition

Opposing emotional events (negative/trauma or positive/maternal care) during the postnatal period may differentially influence vulnerability to the effects of stress later in life. The development and course of intestinal disorders such as inflammatory bowel disease are negatively affected by persistent stress, but to date the role of positive life events on these pathologies has been entirely unknown. In the present study, the effect of early life beneficial experiences in the development of intestinal dysfunctions, where inflammation and stress stimuli play a primary role, was investigated. As a "positive'' experimental model we used adult male rat progeny nursed by mothers whose drinking water was supplemented with moderate doses of corticosterone (CORT) (0.2 mg/ml) during the lactation period. Such animals have been generally shown to cope better with different environmental situations during life. The susceptibility to inflammatory experimental colitis induced by intracolonic infusion of TNBS (2,4,6-trinitrobenzenesulphonic acid) was investigated in CORT-nursed rats in comparison with control rats. This mild increase in maternal corticosterone during lactation induced, in CORT-nursed rats, a long lasting protective effect on TNBS-colitis, characterized by improvements in some indices of the disease (increased colonic myeloperoxidase activity, loss of body weight and food intake) and by the involvement of endogenous peripheral pathways known to participate in intestinal disorder development (lower plasma corticosterone levels and colonic mast cell degranulation, alterations in the colonic expression of both corticotrophin releasing factor/CRF and its receptor/CRH-1R). All these findings contribute to suggesting that the reduced vulnerability to TNBS-colitis in CORT-nursed rats is due to recovery from the colonic mucosal barrier dysfunction. Such long lasting changes induced by mild hormonal manipulation during lactation, making the adult also better adapted to colonic inflammatory stress, constitute a useful experimental model to investigate the etiopathogenetic mechanisms and therapeutic treatments of some gastrointestinal diseases.

Mots clés: corticotropin-releasing-factor; colonic barrier dysfunction; stress-related alterations; childhood abuse; motor function; glucocorticoids; disease

Mots clés: environmental enrichment; receptor; intestine

Domaines: Sciences du Vivant [q-bio]

Carla Petrella, Chiara Giuli, Simona Agostini, Valérie Alquier-Bacquié, Manuela Zinni, et al.. Maternal exposure to low levels of corticosterone during lactation protects against experimental inflammatory colitis-induced damage in adult rat offspring. PLoS ONE, 2014, 9 (11), ⟨10.1371/journal.pone.0113389⟩. ⟨hal-02634271⟩