The immunohistochemicallocalizationoftheglycosphingolipidasialo-GM1in theintestineofweanedpiglets

Iona Trandaburu (1) , Isabelle P. Oswald (2) , Tiberiu Trandaburu (1)

1 Institute of Biology
2 ToxAlim-BioToMyc - Biosynthèse & Toxicité des Mycotoxines

The duodenum,jejunum,ileum,cecumandcolonofthreemalehybridpiglets,4weeksoldjustafterweaning, wereinvestigatedfortheimmunohistochemicallocalizationoftheasialoganglioside,GM1(asialo-GM1).Thestudyrevealedvariousdegreesoflabellingforthisacidglycosphingolipidinneural,epithelialandbloodelementsinallthegutsegments.Theimmunolabelledneuralstructures,representedbyganglionicperikaryaandnervefibers,weredistributedthroughouttheintestinalwalland showedquantitativevariationsinthevariousregions.Incontrastthenumericalevaluationoflabelledepithelialcellswasencounteredonlyintheterminaljejunumandalongtheentireileum,cecum andlargeintestine.Inaddition,aheterogenouspopulationofimmunolabelledleukocyteswasspread randomlyinthelaminapropriaandsubmucosaoftheentireintestineanddidnotshowanyapparent quantitativefluctuationsbetweenthedifferentparts.TheobservationsregardingthetypicaldistributionpatternsoftheasialogangliosideGM1inganglionicperikaryaandepithelialcellsofweanedpigletsarediscussedinrelationtotheirpossiblefunctionalsignificanceintheintestineandothermammalianorgans.

Mots clés: Asialoganglioside GM1; Asialo-GM1; Weanedpiglets; Intestine; Immunohistochemistry

Domaines: Sciences du Vivant [q-bio]

Iona Trandaburu, Isabelle P. Oswald, Tiberiu Trandaburu. The immunohistochemicallocalizationoftheglycosphingolipidasialo-GM1in theintestineofweanedpiglets. Acta Histochemica, 2011, 113, pp.103-108. ⟨10.1016/j.acthis.2009.08.006⟩. ⟨hal-02643287⟩