Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.

Graziela Drociunas Pacheco (1) , Caio Abércio Da Silva (1) , Philippe Pinton (2) , Isabelle P. Oswald (2) , Ana-Paula Loureiro-Bracarense (1)

1 State University of Londrina = Universidade Estadual de Londrina
2 ToxAlim-BioToMyc - Biosynthèse & Toxicité des Mycotoxines

The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed that treatment with 0.5 mM or 1.0 mM phytic acid restores the decrease in TEER caused by 25 μM DON. In conclusion this study demonstrates that phytic acid decreased the negative effects of deoxynivalenol on the membrane integrity of the IPEC-1 intestinal epithelial cell line.

Mots clés: Intestinal cell; Inositol; IP6; Mycotoxin; Toxicity

Domaines: Sciences du Vivant [q-bio]

Graziela Drociunas Pacheco, Caio Abércio Da Silva, Philippe Pinton, Isabelle P. Oswald, Ana-Paula Loureiro-Bracarense. Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.. Experimental and Toxicologic Pathology, 2012, 64 (4), pp.345-7. ⟨10.1016/j.etp.2010.09.008⟩. ⟨hal-02652261⟩