Microstructured liposome array

Barnabé Chaize (1) , Michel Nguyen (2) , Tristan Ruysschaert (1) , Véronique Le Berre (3) , Emmanuelle Trévisiol (3) , Anne-Marie Caminade (2) , Jean Pierre Majoral (2) , Geneviève Pratviel (2) , B. Meunier (2) , Mathias Winterhalter (1) , Didier Fournier (1)

1 IPBS - Institut de pharmacologie et de biologie structurale
2 LCC - Laboratoire de chimie de coordination
3 Biochip and Bionanotechnogy

Conversion of a DNA chip to a nanocapsule array was performed by grafting on a liposome an oligonucleotide complementary to an oligonucleotide bound to the array. Each liposome may be loaded by a soluble molecule or may present a hydrophobic or amphiphilic molecule inserted in its wall. To detect liposomes on the chip, we used fluorescent dyes encapsulated in the liposome internal volume or fluorescent lipids. We observed that an oligonucleotide-grafted liposome containing a defined dye specifically accumulated on the area where its complementary oligonucleotide had been spotted on the array. The virtually unlimited amount of addresses allows the specific binding of large amounts of liposomes in one single batch.

Domaines: Biochimie, Biologie Moléculaire; Biotechnologies

Barnabé Chaize, Michel Nguyen, Tristan Ruysschaert, Véronique Le Berre, Emmanuelle Trévisiol, et al.. Microstructured liposome array. Bioconjugate Chemistry, 2006, 17 (1), pp.245-247. ⟨10.1021/bc050273p⟩. ⟨hal-00120640⟩