Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness

Karine Délécouls-Servat (1) , Alain Bergel (1) , Régine Basséguy (1)

1 LGC - Laboratoire de Génie Chimique

Two membrane electrochemical reactors(MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as electrochemical mediator. A semipermeable membrane (dialysis or ultra-filtration) was integrated in the filter-press electrochemical reactor to confine the enzyme(s) as close as possible to the electrode surface. When methyl viologen was used, the transformation ratio of cyclohexanone varied from 0 to 65% depending on the internal arrangement of the reactor. Matching the reactor configuration to the reaction system was essential in this case. With the rhodium complex, the ultra-filtration MER was tested in continuous and recycling configurations. The best conditions led to 100% transformation of 0.1 L volume of 0.1 M cyclohexanone after 70 h with the recycling mode. Finally, the performances of the reactors are discussed with respect to different evaluations of the production yields.

Mots clés: Electrochemistry; Enzyme catalysis; Membranes; Electrochemical reactor; Synthesis

Domaines: Microbiologie et Parasitologie

Karine Délécouls-Servat, Alain Bergel, Régine Basséguy. Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness. Bioprocess and Biosystems Engineering, 2004, 26, pp.205-215. ⟨10.1007/s00449-004-0356-2⟩. ⟨hal-00450158⟩