Skip to Main content Skip to Navigation
Journal articles

Electroactive cytochromeP450BM3 cast polyion films on graphite electrodes

Abstract : Films of electrochemically active cytochrome P450BM3 were constructed on graphite electrodes using alternate assembly with polyethyleneimin(PEI). The original layer-by-layer adsorption method was slightly modified here to form so-called “cast polyion” films. The cast polyion films were elaborated by immobilizing two successive layers of PEI and protein in very large excess with respect to a monolayer, without any intermediate washing step. Following the immobilization steps by SEM showed that uniform films of a few micrometers were deposited on the graphite surface. The electrochemically activity of the immobilized cytP450 was tested with regard to the reduction of oxygen and the one-electron reduction of the heme. Cyclic voltammetry indicated surface concentration of electrochemically active cytP450 around 0.6 nmol/cm2, which corresponded to 5% of the total amount of protein that was consumed by the immobilisation process. Adapting the procedure to a graphite felt electrode with the view of scaling up porous electrodes for large scale synthesis increased the concentration to 0.9 nmol/cm2. Cast polyion films may represent a simple technique to immobilize high amount of electrochemically active protein, keeping the advantage of the electrostatic interactions of the regular layer-by-layer method.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00475995
Contributor : Open Archive Toulouse Archive Ouverte (oatao) <>
Submitted on : Friday, April 23, 2010 - 1:59:59 PM
Last modification on : Friday, February 5, 2021 - 12:30:02 PM

Identifiers

  • HAL Id : hal-00475995, version 1

Collections

Citation

Aurélie Pardo-Jacques, Régine Basséguy, Alain Bergel. Electroactive cytochromeP450BM3 cast polyion films on graphite electrodes. Electrochimica Acta, Elsevier, 2006, vol. 52 n° 3., 979-987 available on : http://oatao.univ-toulouse.fr/1122/1/Pardo-Jacques_1122.pdf. ⟨hal-00475995⟩

Share

Metrics

Record views

61