Electroactive cytochromeP450BM3 cast polyion films on graphite electrodes

Aurélie Pardo-Jacques (1) , Régine Basséguy (1) , Alain Bergel (1)

1 LGC - Laboratoire de Génie Chimique

Films of electrochemically active cytochrome P450BM3 were constructed on graphite electrodes using alternate assembly with polyethyleneimin(PEI). The original layer-by-layer adsorption method was slightly modified here to form so-called “cast polyion” films. The cast polyion films were elaborated by immobilizing two successive layers of PEI and protein in very large excess with respect to a monolayer, without any intermediate washing step. Following the immobilization steps by SEM showed that uniform films of a few micrometers were deposited on the graphite surface. The electrochemically activity of the immobilized cytP450 was tested with regard to the reduction of oxygen and the one-electron reduction of the heme. Cyclic voltammetry indicated surface concentration of electrochemically active cytP450 around 0.6 nmol/cm2, which corresponded to 5% of the total amount of protein that was consumed by the immobilisation process. Adapting the procedure to a graphite felt electrode with the view of scaling up porous electrodes for large scale synthesis increased the concentration to 0.9 nmol/cm2. Cast polyion films may represent a simple technique to immobilize high amount of electrochemically active protein, keeping the advantage of the electrostatic interactions of the regular layer-by-layer method.

Mots clés: Cytochrome P450BM3; Bioelectrochemistry; Modified electrodes; Cast polyion films; PEI

Domaines: Génie chimique

Aurélie Pardo-Jacques, Régine Basséguy, Alain Bergel. Electroactive cytochromeP450BM3 cast polyion films on graphite electrodes. Electrochimica Acta, 2006, 52 (3), pp.979-987. ⟨10.1016/j.electacta.2006.06.035⟩. ⟨hal-03598078⟩