Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment content

Nabil Majdi (1) , Michèle Tackx (1) , Walter Traunspurger (2) , Evelyne Buffan-Dubau (1)

1 LEFE - Laboratoire Ecologie Fonctionnelle et Environnement
2 Department of Animal Ecology (Morgenbreede, Germany), Germany)

The natural feeding behaviour of the nematodes Chromadorina bioculata (Schultze in Carus 1857) and Chromadorina viridis (Linstow 1876) was studied in situ, within epilithic biofilms of the Garonne River (France). Based on their feeding-type characteristics and population dynamics, it was hypothesized that these species feed selectively on microphytobenthos (MPB) within the biofilm, and that among MPB groups, diatoms are preferred. High-performance liquid chromatography (HPLC) was used for separation, identification and quantification of pigments both in nematode guts and in the biofilm. This is the first time that nematode gut pigment contents were examined under natural conditions. Diatoms dominated the MPB which also comprised cyanobacteria and green microalgae. The comparison between chlorophyll a content in nematode guts versus in the biofilm showed that C. bioculata and C. viridis fed opportunistically (non-selectively) on MPB within the biofilm. Only diatom biomarker pigments were found in nematode guts suggesting that they could preferentially fed on diatoms. However, the non-detection of biomarker pigments for other microphyte groups could be also linked to HPLC detection limits. It was estimated that Chromadorina nematodes daily ingested on average 0.03-0.67% of the MPB standing stock. This grazing covered only a small part of their energetic requirements, suggesting that besides MPB they probably also fed on other biofilm food sources. Some considerations on the applicability of the HPLC gut pigment analysis technique for the examination of nematode feeding are also presented.

Mots clés: Selectivity; Grazing; Diatoms; Periphyton; Meiofauna; Chromadorina

Domaines: Biodiversité; Ecologie, Environnement

Nabil Majdi, Michèle Tackx, Walter Traunspurger, Evelyne Buffan-Dubau. Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment content. Hydrobiologia, 2012, vol. 680, pp. 219-232. ⟨10.1007/s10750-011-0920-0⟩. ⟨hal-00823478⟩