Probing highly selective H/D exchange processes with a ruthenium complex through neutron diffraction and multinuclear NMR studies

Mary Grellier (1) , S.A. Mason (2) , A. Albinati (3) , S.C. Capelli (2) , S. Rizzato (3) , Christian Bijani (1) , Yannick Coppel (1) , Sylviane Sabo-Etienne (1)

1 LCC - Laboratoire de chimie de coordination
2 ILL - Institut Laue-Langevin
3 Department of Biosciences [Milano]

Deuterium labeling is a powerful way to gain mechanistic information in biology and chemistry. However, selectivity is hard to control experimentally, and labeled sites can be difficult to assign both in solution and in the solid state. Here we show that very selective high-deuterium contents can be achieved for the polyhydride ruthenium phosphine complex [RuH2(H2)2(PCyp3)2] (1) (PCyp3 = P(C5H9)3). The selectivity of the H/D exchange process is demonstrated by multinuclear NMR and neutron diffraction analyses. It has also been investigated through density functional theory (DFT) calculations. The reactions are performed under mild conditions at room temperature, and the extent of deuterium incorporation, involving selective C-H bond activation within the cyclopentyl rings of the phosphine ligands, can easily be tuned (solvent effects, D2 pressure). It is shown that D2 gas can inhibit the C-H/C-D exchange process.

Mary Grellier, S.A. Mason, A. Albinati, S.C. Capelli, S. Rizzato, et al.. Probing highly selective H/D exchange processes with a ruthenium complex through neutron diffraction and multinuclear NMR studies. Inorganic Chemistry, 2013, 52 (13), pp.7329-7337. ⟨10.1021/ic302307m⟩. ⟨hal-00995058⟩