Multilevel (3D) microfluidic technology for an innovative magnetic cell separation and couting platform

Marc Fouet (1) , Sébastien Cargou (1) , Rémi Courson (1) , Marie-Charline Blatché (1) , Armelle Montrose (2) , Karine Reybier (2) , Anne Marie Gué (1)

1 LAAS - Laboratoire d'analyse et d'architecture des systèmes
2 PHARMA-DEV - Pharmacochimie et Biologie pour le Développement

We demonstrate a new concept of devices, which by combining 3D fluid engineering and localized mag-netic actuation enables the full integration of a cell tagging and magnetic separation device. We used a low cost, commercially available dry film (EMS Inc, Ohio, USA) that fits microfluidic requirements and gives the possibility to build easily 3D microfluidic structures. The labelling of blood monocytes with su-perparamagnetic particles was performed "up stream" with the aim of a microparticles 3D micromixer while cell separation was achieved "downstream" through continuous vertical separation over integrated micro-coils.

Mots clés: Magnetic separation; Cell tagging; Planar coils; Multilayer (3D)

Domaines: Micro et nanotechnologies/Microélectronique; Sciences du Vivant [q-bio]; Mécanique des fluides [physics.class-ph]

Marc Fouet, Sébastien Cargou, Rémi Courson, Marie-Charline Blatché, Armelle Montrose, et al.. Multilevel (3D) microfluidic technology for an innovative magnetic cell separation and couting platform. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Oct 2014, San Antonio, United States. pp.440-442. ⟨hal-01086517⟩