Effect of dietary pristane and other saturated mineral oils (MOSH) on autoimmune arthritis in rats
Résumé
Pristane and other adjuvants based on mineral oil saturated hydrocarbons (MOSH) may induce autoim-munity in rodents after intradermal injection; however there is a lack of information on immune effectsafter oral MOSH exposure. The aim of our study was to determine the impact of dietary exposure topristane and other MOSH on the development of autoimmune arthritis.Dark Agouti (DA) rats were given feed containing 4000 mg/kg pristane or a broad MOSH mixture invarious concentrations (0–4000 mg/kg) for 90 days, or a single intradermal injection of 200 l pristane(positive control). Arthritis scores, and serum and splenocyte markers previously associated with arthritisdevelopment, were determined.All rats injected with pristane displayed arthritis symptoms and higher levels of certain serum markers.None of the rats fed pristane or MOSH developed arthritis symptoms or demonstrated clear changes inany measured arthritis-associated biological markers in serum or splenocytes.The absence of clinical arthritis symptoms or any increase in common arthritis-associated biologicalmarkers in sera and spleen following dietary exposure to pristane or a broad MOSH mixture in a sub-chronic rat model of arthritis suggest that dietary MOSH have low capacity to promote development ofautoimmunity.