Direct characterization of circulating DNA in blood plasma using µLAS technology

Rémi Malbec (1) , Bayan Chami (1) , H. Ngo (2) , A Didelot (3) , F Garlan (3) , S Garrigou (3) , V Taly (3) , Lorène Aeschbach (4) , Evgeniya Trofimenko (4) , Vincent Dion (4) , Audrey Boutonnet-Rodat (5) , Frédéric Ginot (5) , Aurélien Bancaud (1)

1 LAAS-MILE - Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement
2 HNUE - Hanoi National University of Education
3 UPD5 - Université Paris Descartes - Paris 5
4 UNIL - Université de Lausanne = University of Lausanne
5 Picometrics Technologies

Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the µLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/µL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain.

Domaines: Micro et nanotechnologies/Microélectronique; Ingénierie biomédicale

Rémi Malbec, Bayan Chami, H. Ngo, A Didelot, F Garlan, et al.. Direct characterization of circulating DNA in blood plasma using µLAS technology. 63rd International Electron Devices Meeting (IEDM 2017), Dec 2017, San Francisco, CA, United States. 4p. ⟨hal-01686167⟩