Towards the analysis of mitochondria isolated from leukemic cells with electrochemical instrumented microwell arrays

Gabriel Lemercier (1) , Fadhila Sekli-Belaidi (1) , Venkata Suresh Vajrala (2) , Emeline Descamps (1) , Julie Foncy (3) , Stéphane Arbault (2) , Jean-Emmanuel Sarry (4) , Pierre Temple-Boyer (1) , Jérôme Launay (1)

1 LAAS-MICA - Équipe MICrosystèmes d'Analyse
2 ISM - Institut des Sciences Moléculaires
3 LAAS-ELIA - Équipe Ingénierie pour les sciences du vivant
4 IUCT Oncopole - UMR 1037 - Institut Universitaire du Cancer de Toulouse - Oncopole

This work deals with the development of electrochemical transducers for the analysis of the metabolic status of mitochondria isolated from leukemic cells. It proposes the use of ring nanoelectrodes (RNE) integrated into microwell arrays for the simultaneous monitoring of the oxygen (O2) consumption and the hydrogen peroxide (H2O2) production. The sensor enabled the real-time recording of the oxygen consumption of approximately 10,000 isolated mitochondria. Solutions are now proposed to detect H2O2 production and to reduce the number of mitochondria under test, targeting the single mitochondrion analysis.

Mots clés: Bioelectrochemical nanosensor; microwell arrays; isolated mitochondria; acute myeloid leukemia

Domaines: Micro et nanotechnologies/Microélectronique

Gabriel Lemercier, Fadhila Sekli-Belaidi, Venkata Suresh Vajrala, Emeline Descamps, Julie Foncy, et al.. Towards the analysis of mitochondria isolated from leukemic cells with electrochemical instrumented microwell arrays. EUROSENSORS XXXI 31th European Conference on Solid-State Transducers, Sep 2017, Paris, France. ⟨hal-01687843⟩