pH-CHEMFET-BASED ANALYSIS DEVICES FOR THE BACTERIAL ACTIVITY MONITORING

Marie Laure Pourciel-Gouzy 1 Sandrine Assié-Souleille 2 Laurent Mazenq 3 Jérôme Launay 1 Pierre Temple-Boyer 1
1 LAAS-MICA - Équipe MICrosystèmes d'Analyse
LAAS - Laboratoire d'analyse et d'architecture des systèmes
2 LAAS-I2C - Service Instrumentation Conception Caractérisation
LAAS - Laboratoire d'analyse et d'architecture des systèmes
3 LAAS-TEAM - Service Techniques et Équipements Appliqués à la Microélectronique
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : Silicon and polymer microtechnologies have been developed in order to integrate pH-metry techniques in the frame of medical diagnosis. Thus, a fluidic analysis device has been designed and realised in order to monitor pH-related bacterial activities. It includes a SiO2/Si3N4 pH-sensitive chemical field effect transistor (pH-ChemFET), its titanium/gold pseudo-reference gate electrode and a poly-dimethylsiloxane (PDMS) integrated flow-cell (total volume: ≤ 2 mm 3). The whole analysis device has been used to detect the biological activities of the Lactobacillus Crispatus bacteria and to estimate its sensitivity to antibiotics. Results demonstrate the detection of pH-related bacterial metabolisms in microvolumes, enabling to reduce significantly the analysis response time with regards to standard procedures and to think to the development of pH-ChemFET-metry for medical analysis.
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Marie Laure Pourciel-Gouzy, Sandrine Assié-Souleille, Laurent Mazenq, Jérôme Launay, Pierre Temple-Boyer. pH-CHEMFET-BASED ANALYSIS DEVICES FOR THE BACTERIAL ACTIVITY MONITORING. Sensors and Actuators B: Chemical, Elsevier, 2008, 134 (1), pp.339 - 344. ⟨10.1016/j.snb.2008.04.029⟩. ⟨hal-01687854⟩

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