On the application of surface enhanced Raman scattering to study the interaction of DsRed fluorescent proteins with silver nanoparticles embedded in thin silica layers

Marvine Soumbo (1, 2) , Alessandro Pugliara (1, 3) , Adnen Mlayah (3) , Marie-Carmen Monje (2) , Christine Roques (2) , Bernard Despax (1) , Caroline Bonafos (3) , Robert Carles (3) , Kremena Makasheva (4)

1 LAPLACE - LAboratoire PLasma et Conversion d'Energie
2 LGC - Laboratoire de Génie Chimique
3 CEMES - Centre d'élaboration de matériaux et d'études structurales
4 LAPLACE-MPP - Matériaux et Procédés Plasmas

The interaction of proteins with silver nanoparticles (AgNPs) is of primary importance to uncover silver antimicrobial efficiency and environmental hazard. This interaction can affect silver reactivity, bioavailability and, eventually, silver toxicity towards the environmental media. Detection of the interaction of DsRed fluorescent proteins with AgNPs embedded in thin silica layers is demonstrated using surface enhanced Raman spectroscopy (SERS), but deep analyses require the design and elaboration of dedicated plasmonic substrates giving a high enhancement factor.

Mots clés: Biochemistry; Surface scattering; Substrates; Silica; Enhancement factor; Fluorescence; Metal nanoparticles; Environmental hazards; Surface enhanced Raman Scattering (SERS); Silver nanoparticles (AgNps); Silver nanoparticles; Surface enhanced Raman spectroscopy; Thin silica layers -; Raman scattering; Raman spectroscopy; Fluorescent protein; Environmental media; Silver; DsRed fluorescent proteins; Proteins; Nanotechnology; Nanoparticles

Domaines: Génie chimique; Sciences de l'ingénieur [physics]; Physique [physics]

Marvine Soumbo, Alessandro Pugliara, Adnen Mlayah, Marie-Carmen Monje, Christine Roques, et al.. On the application of surface enhanced Raman scattering to study the interaction of DsRed fluorescent proteins with silver nanoparticles embedded in thin silica layers. 2016 IEEE Nanotechnology Materials and Devices Conference (NMDC), Oct 2016, Toulouse, France. pp.1-2, ⟨10.1109/NMDC.2016.7777162⟩. ⟨hal-01763542⟩