High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.

Roxanne Diaz (1) , Aurore Sanchez (1) , Véronique Le Berre (2) , Jean-Yves Bouet (1)

1 LMGM - Laboratoire de microbiologie et génétique moléculaires - UMR5100
2 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés

Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This methodology, termed ChIP-sequencing (ChIP-seq), enables the identification of the DNA targets of DNA binding proteins across genome-wide maps. Here, we describe the steps necessary to obtain short, specific, high-quality immunoprecipitated DNA prior to DNA library construction for NGS and high-resolution ChIP-seq data.

Mots clés: Affinity-purified antibody; Bacterial nucleoid; Chromatin immunoprecipitation; DNA fragmentation; DNA sonication; DNA target; Genome-wide maps; Next-generation sequencing

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire

Roxanne Diaz, Aurore Sanchez, Véronique Le Berre, Jean-Yves Bouet. High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.. Methods in Molecular Biology, 2018, pp.61-73. ⟨10.1007/978-1-4939-7098-8_6⟩. ⟨hal-01849465⟩