Toward the analysis of mitochondria isolated from leukemic cells with electrochemically instrumented microwell arrays

Gabriel Lemercier (1) , Fadhila Sekli-Belaidi (1) , Venkata S Vajrala (2) , Emeline Descamps (1) , N. Sojic (2) , Stéphane Arbault (2) , Jean Emmanuel E Sarry (3) , Pierre Temple-Boyer (1) , Jérôme Launay (1)

1 LAAS-MICA - Équipe MICrosystèmes d'Analyse
2 ISM - Institut des Sciences Moléculaires
3 CRCT - Centre de Recherches en Cancérologie de Toulouse

This work deals with the development of electrochemical transducers for the analysis of the metabolic status of mitochondria isolated from leukemic cells. It proposes the use of ring nanoelectrodes (RNE) integrated into microwell arrays for the simultaneous monitoring of the oxygen (O2) consumption and the hydrogen peroxide (H2O2) production. The sensor enabled the real-time recording of the oxygen consumption of approximately 10,000 isolated mitochondria. Solutions are now proposed to detect H2O2 production and to reduce the number of mitochondria under test, targeting the single mitochondrion analysis.

Domaines: Micro et nanotechnologies/Microélectronique

Gabriel Lemercier, Fadhila Sekli-Belaidi, Venkata S Vajrala, Emeline Descamps, N. Sojic, et al.. Toward the analysis of mitochondria isolated from leukemic cells with electrochemically instrumented microwell arrays. 19th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2017), Jun 2017, Kaohsiung, Taiwan. 4p., ⟨10.1109/TRANSDUCERS.2017.7994374⟩. ⟨hal-01871405⟩