Dynamic model for isopropanol production by Cupriavidus necator

Céline Ternon (1, 2) , Estelle Grousseau (1, 3) , Jan Gunther (1, 3) , Nathalie Gorret (1, 3) , Stéphane Guillouet (1, 3) , A. J. Sinskey (4, 5, 6) , Cesar Arturo Aceves-Lara (1, 3) , Gilles Roux (7, 2, 1)

1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 LAAS-DISCO - Équipe DIagnostic, Supervision et COnduite
3 UT - Université de Toulouse
4 Engineering Systems Division [Cambridge , USA]
5 Department of Biology
6 Division of Health Sciences and Technology (HST)
7 INSA Toulouse - Institut National des Sciences Appliquées - Toulouse

The Hybrid Cybernetic Model (HCM) enables the simulation of metabolic fluxes by using Elementary Modes Analysis and taking into account of selected cellular regulations. These latter are represented by cybernetic control variables. In this study, a simplified metabolic network was established in order to isolate a subset of Elementary Modes, representative of the main phenotypic capabilities of the microorganism. An innovative classification of the modes was introduced in the dynamic model, which permitted the selection of the active modes based on the microbial kinetics. The case study presented here is a genetically modified strain of Cupriavidus necator, engineered to produce isopropanol. Available experimental data were used for identification of parameters in the dynamic model. This model can be used in order to predict the value of maximal and minimal product yields when other substrates will be tested.

Mots clés: Biofuel; Yield Analysis; Isopropanol production; Elementary Modes (EMs); Hybrid Cybernetic Model (HCM)

Domaines: Sciences du Vivant [q-bio]

Céline Ternon, Estelle Grousseau, Jan Gunther, Nathalie Gorret, Stéphane Guillouet, et al.. Dynamic model for isopropanol production by Cupriavidus necator. 19th World Congress of the International-Federation-of-Automatic-Control, Aug 2014, Cape Town, South Africa. pp.6, ⟨10.3182/20140824-6-ZA-1003.02267⟩. ⟨hal-03628031⟩