In-situ viscometry during hydrolysis of lignocellulosic materials by single and cocktail enzymatic activities: from material impact to viscosity overshoot

Tuan Le (1, 2, 3) , Dominique Anne-Archard (4, 2) , Véronique Coma (5) , Xavier Cameleyre (1) , Eric Lombard (1) , Kim Anh To (3) , Tuan Anh Pham (3) , Tien Cuong Nguyen (1, 3) , Luc Fillaudeau (1, 2)

1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 FERMAT - Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts
3 Center for Research and Development in Biotechnology (CRDB), Schools of Biotechnology and Food Technology (SBFT)
4 IMFT - Institut de mécanique des fluides de Toulouse
5 LCPO - Laboratoire de Chimie des Polymères Organiques

Rheological characterization of lignocellulosic suspension is a fundamental research to overpass physical limitations during enzymatic hydrolysis. In this study, the rheological properties of lignocellulosic suspensions (filter paper - FP, paper pulp - PP and pretreated sugarcane bagasse - SCB) and their evolution during enzymatic hydrolysis were investigated by in-situ measurements. All tested suspensions behaved as shear thinning fluids and a critical concentration reflecting the change between diluted to semi-dilute regimes were identified.

Mots clés: lignocellulose substrate; in-situ rheometry; hydrolysis; pure activity; enzymatic cocktail

Domaines: Sciences du Vivant [q-bio]

Tuan Le, Dominique Anne-Archard, Véronique Coma, Xavier Cameleyre, Eric Lombard, et al.. In-situ viscometry during hydrolysis of lignocellulosic materials by single and cocktail enzymatic activities: from material impact to viscosity overshoot. 16. Congrès de la Société Française de Génie des Procédés "Le Génie des Procédés au Service de l'Homme" (SFGP 2017), Jul 2017, Nancy, France. 1054 p. ⟨hal-01886476⟩