Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species

Maha Mehanna (1) , Ingrid Rouvre (1) , Marie-Line Délia-Dupuy (1) , Damien Féron (2) , Alain Bergel (1) , Régine Basséguy (1)

1 LGC - Laboratoire de Génie Chimique
2 CEA - Commissariat à l'énergie atomique et aux énergies alternatives

Mild steel coupons were exposed to hydrogenase in a 10 mM phosphate solution. Control coupons were covered by a layer of vivianite. The injection of hydrogenase caused a fast increase in the open circuit potential; this increase depended on the amount of hydrogenase injected and increased from 8 mV for 30 μL hydrogenase to 63 mV for 80 μL. The presence of enzyme resulted in a thicker deposit: high amounts induced the accumulation of corrosion products. Hydrogenase that was deactivated by air revealed a protective effect: non-degradation was observed. In contrast, hydrogenase that was denatured by heat provoked an important deposit of corrosion products with a heterogeneous, cracked structure. The study showed that the action of hydrogenase is not linked to its regular enzymatic activity but to a balance between the protective effect of its protein shell and the electrochemical action of its iron-sulphur clusters. Depending on the operating conditions, hydrogenase can either enhance or mitigate the formation of a corrosion layer on mild steel.

Mots clés: Hydrogenase; Mild steel; Phosphate medium; Microbial corrosion; Microbially influenced corrosion

Domaines: Génie chimique; Génie des procédés

Maha Mehanna, Ingrid Rouvre, Marie-Line Délia-Dupuy, Damien Féron, Alain Bergel, et al.. Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species. Bioelectrochemistry, 2016, 111, pp.31-40. ⟨10.1016/j.bioelechem.2016.04.005⟩. ⟨hal-01907323⟩