Impact of inhibitory peptides released by Saccharomyces cerevisiae BDX on the malolactic fermentation performed by Oenococcus oeni Vitilactic F

Ziad Rizk (1, 2, 3) , Youssef El Rayess (2) , Chantal Ghanem (1, 2) , Florence Mathieu (3) , Patricia Taillandier (3) , Nancy Nehme (2, 4)

1 LARI - Lebanese Agricultural Research Institute
2 Holy-Spirit University of Kaslik [Jounieh]
3 LGC - Laboratoire de Génie Chimique
4 LU / ULB - الجامعة اللبنانية [بيروت] = Lebanese University [Beirut] = Université libanaise [Beyrouth]

A previous study has shown that the malolactic fermentation (MLF) was inhibited during sequential fermentations performed with the pair Saccharomyces cerevisiae BDX/Oenococcus oeni Vitilactic F in synthetic grape juices. A yeast peptidic fraction with an apparent MW of 5-10kDa was involved in the inhibition. In the present study, the MLF was also inhibited in Cabernet Sauvignon and Syrah wines. The inhibition due to the peptidic fraction was maintained despite high phenolic contents. Kinetic studies showed that the peptidic fraction was gradually released during the alcoholic fermentation (AF). Its highest anti-MLF effect was reached when isolated from late stages of the AF stationary phase. The peptidic fraction was tested in vitro on cell-free bacterial cytosolic extracts containing the malolactic enzyme in a pH range between 3.5 and 6.7. Results showed that it was able to directly inhibit the malolactic enzyme activity with an increasing inhibitory kinetic correlated to the AF time at which it was collected.

Mots clés: Antibacterial yeast peptidic compounds; Malolactic enzyme; Oenococcus oeni; Saccharomyces cerevisiae

Domaines: Génie chimique; Génie des procédés

Ziad Rizk, Youssef El Rayess, Chantal Ghanem, Florence Mathieu, Patricia Taillandier, et al.. Impact of inhibitory peptides released by Saccharomyces cerevisiae BDX on the malolactic fermentation performed by Oenococcus oeni Vitilactic F. International Journal of Food Microbiology, 2016, 233, pp.90-96. ⟨10.1016/j.ijfoodmicro.2016.06.018⟩. ⟨hal-01907324⟩