Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells

Amine Yekkour (1, 2, 3) , Daniel Tran (1) , Delphine Arbelet-Bonnin (1) , Joël Briand (1) , Florence Mathieu (4) , Ahmed Lebrihi (4, 5) , Rafik Errakhi (5) , Nasserdine Sabaou (2) , François Bouteau (1)

1 LIED (UMR_8236) - Laboratoire Interdisciplinaire des Energies de Demain
2 Laboratoire de Biologie des Systèmes Microbiens - LBSM (Alger, Algeria)
3 INRAA - Institut National de la Recherche Agronomique d'Algérie
4 LGC - Laboratoire de Génie Chimique
5 UMI - جامعة مولاي إسماعيل = Université Moulay Ismaïl

Deoxynivalenol (DON) is a mycotoxin affecting animals and plants. This toxin synthesized by Fusarium culmorum and Fusarium graminearum is currently believed to play a decisive role in the fungal phytopathogenesis as a virulence factor. Using cultured cells of Nicotiana tabacum BY2, we showed that DON-induced programmed cell death (PCD) could require transcription and translation processes, in contrast to what was observed in animal cells. DON could induce different cross-linked pathways involving (i) reactive oxygen species (ROS) generation linked, at least partly, to a mitochondrial dysfunction and a transcriptional down-regulation of the alternative oxidase (Aox1) gene and (ii) regulation of ion channel activities participating in cell shrinkage, to achieve PCD.

Mots clés: Programmed cell death; Deoxynivalenol; Fusarium; Nicotiana tabacum; Ion channels

Domaines: Génie chimique; Génie des procédés

Amine Yekkour, Daniel Tran, Delphine Arbelet-Bonnin, Joël Briand, Florence Mathieu, et al.. Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells. Plant Science, 2015, 238, pp.148-157. ⟨10.1016/j.plantsci.2015.06.004⟩. ⟨hal-01917337⟩