Binding of poly(amidoamine), carbosilane, phosphorus and hybrid dendrimers to thrombin-Constants and mechanisms

D. Shcharbin (1) , E. Pedziwiatr-Werbicka (2) , Aliaksandra Vcherashniaya (3) , A. Janaszewska (2) , Monika Marcinkowska (2) , Piotr Goska (2) , B. Klajnert-Maculewicz (2) , M. Ionov (2) , Viktar Abashkin (1) , Aliaksei Ihnatsyeu-Kachan (1) , F. J. de La Mata (4, 5) , Paula Ortega (4, 5) , R. Gomez-Ramirez (4, 5) , Jean Pierre Majoral (6) , M. Bryszewska (2)

1 Institute of Biophysics and Cell Engineering, NASB, Minsk
2 Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz
3 Department of Biophysics, Belarusian State University
4 Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares,
5 CIBER-BBN - Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
6 LCC - Laboratoire de chimie de coordination

Thrombin is an essential part of the blood coagulation system; it is a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, and catalyzes many other coagulation-related reactions. Absorption at its surface of small nanoparticles can completely change the biological properties of thrombin. We have analyzed the influence on thrombin of 3 different kinds of small nanoparticles: dendrimers (phosphorus-based, carbosilane based and polyamidoamine) and 2 hybrid systems containing carbosilane, viologen and phosphorus dendritic scaffolds in one single molecule, bearing different flexibility, size and surface charge. There was significant alteration in the rigidity of the rigid dendrimers in contrast to flexible dendrimers. These differences in their action are important in understanding interactions taking place at a bio-nanointerface.

Domaines: Chimie de coordination

D. Shcharbin, E. Pedziwiatr-Werbicka, Aliaksandra Vcherashniaya, A. Janaszewska, Monika Marcinkowska, et al.. Binding of poly(amidoamine), carbosilane, phosphorus and hybrid dendrimers to thrombin-Constants and mechanisms. Colloids and Surfaces B: Biointerfaces, 2017, 155, pp.11-16. ⟨10.1016/j.colsurfb.2017.03.053⟩. ⟨hal-01945627⟩