Label-free discrimination of human lymphoma cell sub-populations with microwave dielectric spectroscopy

Katia Grenier (1) , François Artis (1) , Mary Poupot (2) , J-J Fournié (2) , David Dubuc (1)

1 LAAS-MH2F - Équipe Micro et nanosystèmes HyperFréquences Fluidiques
2 CRCT - Centre de Recherches en Cancérologie de Toulouse

This paper deals with the label-free microwave die-lectric characterization of B lymphoma cell lines, the OCI-LY7, OCI-LY10 and OCI-LY18 ones, and demonstrates that the technique is able to discriminate these three sub-population cell lines with their different dielectric properties. Proof is given that such a differentiation is not due to a variation of cell size, viability or cells concentration, but to internal molecular differences. This constitutes a major result as it demonstrates for the first time that microwave dielectric spectroscopy is sufficiently sensitive using di-electric properties variations to distinguish very similar biological cell lines of the same phenotype, B lymphoma cell lines in our case, whereas such a detection is traditionally performed with marker-based and costly equipment or using heavy genetic methods.

Mots clés: biological cells; biosensor; lymphoma; permittivity; sub-populations; Microwave dielectric spectroscopy

Domaines: Electromagnétisme; Micro et nanotechnologies/Microélectronique; Imagerie

Katia Grenier, François Artis, Mary Poupot, J-J Fournié, David Dubuc. Label-free discrimination of human lymphoma cell sub-populations with microwave dielectric spectroscopy. IEEE International Microwave Symposium (IMS 2018), Jun 2018, Philadelphie, PA, United States. 4p. ⟨hal-01951446⟩