Short term exposure to multi-walled carbon nanotubes induce oxidative stress and DNA damage in Xenopus laevis tadpoles

Rayenne Saria (1) , Florence Mouchet (1, 2) , Annie Perrault (1) , Emmanuel Flahaut (2, 3) , Christophe Laplanche (1) , Jean-Charles Boutonnet (2, 4) , Eric Pinelli (1, 2) , Laury Gauthier (1, 2)

1 LEFE - Laboratoire Ecologie Fonctionnelle et Environnement
2 NAnotUbes et écoToxIcoLogiE - NAUTILE (France)
3 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
4 Arkema - Arkema

The potential impact of Multiwalled Carbon NanoTubes (MWCNTs) was investigated on Xenopus laevis tadpoles exposed to 0.1, 1 and 10mg/L. Oxidative stress was measured in entire larvae exposed and DNA damage (Comet assay) was carried out in erythrocytes of circulating blood from 2h to 24h according to standardized recommendations. Results showed significant H2O2 production when larvae were exposed to 1mg/L and 10mg/L of MWCNTs after 4h and 2h of exposure, respectively. Antioxidant enzyme activities showed significant induction of catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) from only 2h of exposure to 10mg/L of MWCNTs. In presence of 1mg/L of MWCNTs, only GR and CAT activities were significantly induced at 4h. Enzyme activities do not follow a simple dose-effect relation, but the time of induction is shortened in relation with the tested concentration. The Comet assay results showed significant DNA damages with a dose dependent response. The profiles of DNA damages show fluctuations, in course of time, which are characteristics of oxidative stress response in relation with the continuous balance between damage and compensation process.

Mots clés: Amphibian; Glutathione reductase; Superoxide dismutase; Catalase; Nanoparticles; Comet assay

Domaines: Génie chimique; Ingénierie de l'environnement; Génie des procédés

Rayenne Saria, Florence Mouchet, Annie Perrault, Emmanuel Flahaut, Christophe Laplanche, et al.. Short term exposure to multi-walled carbon nanotubes induce oxidative stress and DNA damage in Xenopus laevis tadpoles. Ecotoxicology and Environmental Safety, 2014, 107, pp.22-29. ⟨10.1016/j.ecoenv.2014.05.010⟩. ⟨hal-01963106⟩