Nanofluidic fluorescence microscopy with integrated concentration gradient generation for one-shot parallel kinetic assays

Pattamon Teerapanich (1) , Martine Pugniere (2) , Corinne Henriquet (2) , Yii-Lih Lin (3) , Antoine Naillon (4) , Pierre Joseph (4) , Chia-Fu Chou (3) , Thierry Leichle (1)

1 LAAS-MEMS - Équipe Microsystèmes électromécaniques
2 IRCM - U1194 Inserm - UM - Institut de Recherche en Cancérologie de Montpellier
3 Academia Sinica
4 LAAS-MILE - Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement

We report a simple and cost-effective nanofluidic fluorescence microscopy platform with parallel kinetic assay capability for the determination of kinetic parameters in a single run. An on-chip microfluidic concentration diluter, or gradient generator, was integrated to a biofunctionalized nanofluidic chip, enabling simultaneous interrogation of multiple biomolecular interactions with a full titration series of analyte in a single experiment. We demonstrate that since the association and dissociation phases are induced by the on-chip gradient generator and a reverse buffer flow operation, complete kinetic sensorgrams for IgG/anti-IgG interactions can be achieved within 20 min on a single device, which is at least 10 times faster than traditional kinetic techniques. This method could contribute to low-cost, rapid and high-throughput drug-screening and clinical diagnostics.

Domaines: Micro et nanotechnologies/Microélectronique

Pattamon Teerapanich, Martine Pugniere, Corinne Henriquet, Yii-Lih Lin, Antoine Naillon, et al.. Nanofluidic fluorescence microscopy with integrated concentration gradient generation for one-shot parallel kinetic assays. Sensors and Actuators B: Chemical, 2018, 274, pp.338-342. ⟨10.1016/j.snb.2018.07.167⟩. ⟨hal-01964831⟩