Expression of pyrrothineN-acyltransferase activities inSaccharothrix algeriensisNRRL B-24137: new insights into dithiolopyrrolone antibiotic biosynthetic pathway

Anne-Claire Chorin (1) , Laurent Bijeire (2) , Marie-Carmen Monje (1) , Geneviève Baziard (2) , Ahmed Lebrihi (1) , Florence Mathieu (1)

1 LGC - Laboratoire de Génie Chimique
2 Faculté des Sciences Pharmaceutiques

The hypothetical dithiolopyrrolone biosynthetic pathway includes a final step of pyrrothine nucleus acylation. The presence of an enzymatic activity catalysing this reaction was investigated in Saccharothrix algeriensis NRRL B‐24137. To understand the effect exerted by organic acids on the level of dithiolopyrrolone production, their influence on enzymatic expression was studied. Methods and Results: The transfer of acetyl‐CoA or benzoyl‐CoA on pyrrothine was assayed in the cell‐free extract of Sa. algeriensis NRRL B‐24137. This study reports the presence of an enzymatic activity catalysing this reaction that was identified as either pyrrothine N‐acetyltransferase or N‐benzoyltransferase. The stimulation of benzoyl‐pyrrothine (BEP) production by addition of benzoic acid at 1·25 mmol l−1 into the culture medium was demonstrated, and results showed that under the same conditions of growth, pyrrothine N‐benzoyltransferase specific activity was doubled. This study shows that BEP production is enhanced in the presence of benzoic acid partly because of an induction of pyrrothine N‐benzoyltransferase. S

Mots clés: Antibiotic synthases regulation; Dithiolopyrrolone biosynthesis; Saccharothrix algeriensis

Domaines: Biotechnologies; Génie des procédés

Anne-Claire Chorin, Laurent Bijeire, Marie-Carmen Monje, Geneviève Baziard, Ahmed Lebrihi, et al.. Expression of pyrrothineN-acyltransferase activities inSaccharothrix algeriensisNRRL B-24137: new insights into dithiolopyrrolone antibiotic biosynthetic pathway. Journal of Applied Microbiology, 2009, 107 (5), pp.1751-1762. ⟨10.1111/j.1365-2672.2009.04496.x⟩. ⟨hal-02066563⟩