HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza (1) , Carmen López (1) , Josep López-Santín (1) , Cheikhou Kane (2) , M. Dolors Benaiges (1) , Théodore Tzedakis (3)

1 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
2 UCAD - Université Cheikh Anta Diop de Dakar [Sénégal]
3 LGC - Laboratoire de Génie Chimique

This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

Mots clés: Electrochemical NAD+ regeneration; Electrochemical microreactor; Horse-liver alcohol dehydrogenase; β-amino acids; β-alanine synthesis; Simulation

Domaines: Génie chimique; Autre; Catalyse

Rossmery A. Rodríguez-Hinestroza, Carmen López, Josep López-Santín, Cheikhou Kane, M. Dolors Benaiges, et al.. HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor. Chemical Engineering Science, 2017, 158, pp.196-207. ⟨10.1016/j.ces.2016.10.010⟩. ⟨hal-02134833⟩