A direct and simple method for rapidly counting viable chains of Leuconostoc in batch cultures

Florence Plihon (1) , Patricia Taillandier (1) , Pierre Strehaiano (1)

1 LGC - Laboratoire de Génie Chimique

Plating determines the concentration in viable cells of bacteria but remains time-consuming and inaccurate. A direct and simple method combining two techniques, haemacytometry and epifluorescence, gives both the viability and the concentration in viable cells. The method was evaluated using Leuconostoc mesenteroides.

Mots clés: Viable cell; Batch culture; Leuconostoc mesenteroides

Domaines: Génie chimique; Génie des procédés

Florence Plihon, Patricia Taillandier, Pierre Strehaiano. A direct and simple method for rapidly counting viable chains of Leuconostoc in batch cultures. Biotechnology Techniques, 1995, 9 (6), pp.451-456. ⟨10.1007/BF00160835⟩. ⟨hal-02143232⟩