Influence of medium composition, pH and temperature on the growth and viability of Lactobacillus acidophilus

Patricia Taillandier (1) , Jean-François Gilis (1) , Felipe Ramon-Portugal (1) , Patrice Laforce (2) , Pierre Strehaiano (1)

1 LGC - Laboratoire de Génie Chimique
2 Lallemand SAS

The Lactobacillus acidophilus growth was investigated to find the optimal concentrations of 2 nitrogen sources (thought to be in excess) and 2 organic acids. A Plackctt and Burman experimental design was used allowing identification of the more important factors with very few experiments. In the studied range, all the factors had a linear effect and a great influence on the final viability. 20 g/L of each nitrogen sources, 3 g sodium-citrate/L and 5 g sodium acetate/L are necessary. pH, temperature and glucose had poor influence.

Mots clés: Nitrogen; Glucose; Sodium; Organic Chemistry; Organic Acid

Domaines: Génie chimique; Génie des procédés

Patricia Taillandier, Jean-François Gilis, Felipe Ramon-Portugal, Patrice Laforce, Pierre Strehaiano. Influence of medium composition, pH and temperature on the growth and viability of Lactobacillus acidophilus. Biotechnology Letters, 1996, 18 (7), pp.775-780. ⟨10.1007/BF00127887⟩. ⟨hal-02143238⟩