Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

Phuong Lan Pham (1) , Patricia Taillandier (1) , Michel Delmas (2) , Pierre Strehaiano (1)

1 LGC - Laboratoire de Génie Chimique
2 Ecole Nationale Supérieure de Chimie de Toulouse

The concentrations of oat spelt xylan, casein hydrolysate and NH4Cl in the culture medium for production of xylanase from Bacillus sp. I-1018 were optimized by means of response surface methods. The path of steepest ascent was used to approach the optimal region of the medium composition. The optimum composition of the nutrient medium was then easily determined by using a central composite design and was found to be 3.16g/l of xylan, 1.94g/l casein hydrolysate, 0.8g/l of NH4Cl. The xylanase production was increased by 135% when the strain was grown in the optimized medium compared to initial medium.

Mots clés: Bacillus sp. I-1018; Central composite design; Full factorial design; Path of steepest ascent; Xylanase

Domaines: Génie chimique; Génie des procédés

Phuong Lan Pham, Patricia Taillandier, Michel Delmas, Pierre Strehaiano. Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs. World Journal of Microbiology and Biotechnology, 1997, 14 (2), pp.185-190. ⟨10.1023/A:1008821827445⟩. ⟨hal-02143245⟩