Adipose Stem Cells (ASCs) isolation by on-chip pre-treatment of biological samples

Marion Valette (1, 2, 3) , Mathias Bouguelmouna (1, 2) , Mélanie Mariotte (1, 4) , Rémi Courson (5) , Marie-Charline Blatché (6) , Amandine Girousse (7) , Coralie Sengenes (7) , Karine Reybier (3) , Anne Marie Gué (1)

1 LAAS-MILE - Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement
2 STROMALab
3 PHARMA-DEV - Pharmacochimie et Biologie pour le Développement
4 Faculté des Sciences et Techniques de Limoges
5 LAAS-TEAM - Service Techniques et Équipements Appliqués à la Microélectronique
6 LAAS-I2C - Service Instrumentation Conception Caractérisation
7 UT3 - Université Toulouse III - Paul Sabatier

This study presents the development of an original 2-steps Lab-on-chip that aims at isolating Adipose Stem Cells (ASCs), cells of considerable interest for regenerative medicine, from complex biological samples. Based on hydrodynamic filtration, the device pre-isolates ASCs by removing cells with a diameter below 10µm such as red blood cells or lymphocytes.

Mots clés: Adipose Stem Cells; Hydrodynamic Filtration; Stem Cells; Dry Films; Microfluidics

Domaines: Mécanique des fluides [physics.class-ph]; Mécanique des fluides [physics.class-ph]; Micro et nanotechnologies/Microélectronique; Biotechnologies

Marion Valette, Mathias Bouguelmouna, Mélanie Mariotte, Rémi Courson, Marie-Charline Blatché, et al.. Adipose Stem Cells (ASCs) isolation by on-chip pre-treatment of biological samples. Lab-On-Chip and Microfluidics Europe, Jun 2019, Rotterdam, Netherlands. 2019. ⟨hal-02190393⟩