Influence of Homogenization Technique and Blend Ratio on Chitosan/Alginate Polyelectrolyte Complex Properties

Marion Castel (1) , Guillaume Conzatti (1) , Jérôme Torrisani (2) , Antoine Rouilly (2) , Sandrine Cavalie (1) , Nicolas Carrere (2) , Audrey Tourrette (1)

1 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
2 CAI - Chimie Agro-Industrielle

Polyelectrolyte complex (PEC) films were prepared from chitosan (CHI) and alginate (ALG) which are polymers of opposite charge. Two homogenization techniques and two ratios of ALG–CHI blends were compared: mechanical agitation under vacuum (ALG–CHI ST) or agitation by high turbulence (ALG–CHI UT) and 50/50 or 63/37 ratios. Surface and structure of PEC films are affected by the homogenization technique while the swelling percentage is only affected by polymer ratio. The homogenization ratio does not seem to influence in vitro cell proliferation. Results show that the UT homogenization technique with a 63/37 ratio, which gives films with a smooth, homogeneous surface and a higher rate of enzymatic resistance, is more efficient for cell proliferation and viability. These first results confirm the potential use of ALG–CHI films for surgery application.

Mots clés: Alginate; Chitosan; Polyelectrolyte complex; Film

Domaines: Biomatériaux; Polymères; Matériaux

Marion Castel, Guillaume Conzatti, Jérôme Torrisani, Antoine Rouilly, Sandrine Cavalie, et al.. Influence of Homogenization Technique and Blend Ratio on Chitosan/Alginate Polyelectrolyte Complex Properties. Journal of Medical and Biological Engineering, 2018, 38 (1), pp.10-21. ⟨10.1007/s40846-017-0304-7⟩. ⟨hal-02336123⟩