Sources and reservoirs of Staphylococcus capitis NRCS-A inside a NICU

Marine Butin (1, 2) , Yann Dumont (1) , Alice Monteix (1) , Aurane Raphard (1) , Christine Roques (3) , Patricia Martins Simões (1, 2) , Jean-Charles Picaud (1, 4) , Frédéric Laurent (1, 5, 2)

1 HCL - Hospices Civils de Lyon
2 StaPath - Pathogénie des Staphylocoques – Staphylococcal Pathogenesis
3 LGC - Laboratoire de Génie Chimique
4 CarMeN - Cardiovasculaire, métabolisme, diabétologie et nutrition
5 UCBL ISPB - Université Claude Bernard Lyon 1 - Institut des Sciences Pharmaceutiques et Biologiques

Background: The methicillin-resistant clone Staphylococcus capitis NRCS-A, involved in sepsis in neonatal intensive care units (NICUs) worldwide, is able to persist and spread in NICUs, suggesting the presence of reservoirs inside each setting. The purpose of the present study was to identify these reservoirs and to investigate the cycle of transmission of NRCS-A in one NICU. Methods: In a single institution study, NRCS-A was sought in 106 consecutive vaginal samples of pregnant women to identify a potential source of NRCS-A importation into the NICU. Additionally NICU caregivers and environmental including incubators were tested to identify putative secondary reservoirs. Finally, the efficacy of disinfection procedure in the elimination of NRCS-A from incubators was evaluated. Results: No S. capitis was isolated from vaginal samples of pregnant women. Three of the 21 tested caregivers (14%) carried S. capitis on their hands, but none remain positive after a five-day wash-out period outside NICU. Moreover, the clone NRCS-A persisted during six consecutive weeks in the NICU environment, but none of the sampled sites was constantly contaminated. Finally in our before/after disinfection study, all of 16 incubators were colonized before disinfection and 10 (62%) incubators remained colonized with NRCS-A after the disinfection procedure. Conclusions: The partial ineffectiveness of incubators' disinfection procedures is responsible for persistence of NRCS-A inside a NICU, and the passive hand contamination of caregivers could be involved in the inter-patient transmission of S. capitis.

Mots clés: Sepsis; Staphylococcus capitis; Environment; Incubators; Neonatal ICUs; Nrcs-a

Domaines: Sciences du Vivant [q-bio]

Marine Butin, Yann Dumont, Alice Monteix, Aurane Raphard, Christine Roques, et al.. Sources and reservoirs of Staphylococcus capitis NRCS-A inside a NICU. Antimicrobial Resistance and Infection Control, 2019, 8 (1), pp.157. ⟨10.1186/s13756-019-0616-1⟩. ⟨hal-02466555⟩