Genetic Control of Water Use Efficiency and Leaf Carbon Isotope Discrimination in Sunflower (Helianthus annuus L.) Subjected to Two Drought Scenarios

Afifuddin Adiredjo , Olivier Navaud (1) , Stephane Munos (2) , Nicolas Langlade (2) , Thierry Lamaze (1) , Philippe Grieu (3)

1 CESBIO - Centre d'études spatiales de la biosphère
2 Unité mixte de recherche interactions plantes-microorganismes
3 AGIR - AGroécologie, Innovations, teRritoires

High water use efficiency (WUE) can be achieved by coordination of biomass accumulation and water consumption. WUE is physiologically and genetically linked to carbon isotope discrimination (CID) in leaves of plants. A population of 148 recombinant inbred lines (RILs) of sunflower derived from a cross between XRQ and PSC8 lines was studied to identify quantitative trait loci (QTL) controlling WUE and CID, and to compare QTL associated with these traits in different drought scenarios. We conducted greenhouse experiments in 2011 and 2012 by using 100 balances which provided a daily measurement of water transpired, and we determined WUE, CID, biomass and cumulative water transpired by plants. Wide phenotypic variability, significant genotypic effects, and significant negative correlations between WUE and CID were observed in both experiments. A total of nine QTL controlling WUE and eight controlling CID were identified across the two experiments. A QTL for phenotypic response controlling WUE and CID was also significantly identified. The QTL for WUE were specific to the drought scenarios, whereas the QTL for CID were independent of the drought scenarios and could be found in all the experiments. Our results showed that the stable genomic regions controlling CID were located on the linkage groups 06 and 13 (LG06 and LG13). Three QTL for CID were co-localized with the QTL for WUE, biomass and cumulative water transpired. We found that CID and WUE are highly correlated and have common genetic control. Interestingly, the genetic control of these traits showed an interaction with the environment (between the two drought scenarios and control conditions). Our results open a way for breeding higher WUE by using CID and marker-assisted approaches and therefore help to maintain the stability of sunflower crop production.

Mots clés: TRANSPIRATION EFFICIENCY; ARABIDOPSIS-THALIANA; QTL ANALYSIS; WHOLE-PLANT; PHOTOSYNTHETIC CAPACITY; BIOMASS PRODUCTIVITY

Mots clés: QUANTITATIVE TRAIT LOCI; ENVIRONMENT INTERACTIONS; STOMATAL CONDUCTANCE; GENOTYPIC VARIATION

Domaines: Sciences du Vivant [q-bio]; Biologie végétale; Sciences de l'Homme et Société; Sciences de l'environnement

Afifuddin Adiredjo, Olivier Navaud, Stephane Munos, Nicolas Langlade, Thierry Lamaze, et al.. Genetic Control of Water Use Efficiency and Leaf Carbon Isotope Discrimination in Sunflower (Helianthus annuus L.) Subjected to Two Drought Scenarios. PLoS ONE, 2014, 9 (7), ⟨10.1371/journal.pone.0101218⟩. ⟨hal-02640496⟩