Viable skin efficiently absorbs and metabolizes bisphenol A

Daniel Zalko (1) , Carine Jacques (2) , Hélène Duplan (3) , Sandrine S. Bruel (1) , Elisabeth Perdu (2)

1 ToxAlim-MeX - Métabolisme et Xénobiotiques
2 ToxAlim - ToxAlim
3 LABORATOIRE DE PHARMACOCINéTIQUE CUTANéE - Laboratoire de Pharmacocinétique Cutanée

Skin contact has been hypothesized to contribute to human exposure to bisphenol A (BPA). We examinedthe diffusion and metabolism of BPA using viable skin models: human skin explants and short-term culturesof pig ear skin, an alternative model for the study of the fate of xenobiotics following contact exposure.14C-BPA [50–800 nmol] was applied on the surface of skin models. Radioactivity distribution wasmeasured in all skin compartments and in the diffusion cells of static cells diffusion systems. BPA andmetabolites were further quantified by radio-HPLC. BPA was efficiently absorbed in short-term cultures,with no major difference between the models used in the study [viable pig ear skin: 65%; viable humanexplants: 46%; non-viable (previously frozen) pig skin: 58%]. BPA was extensively metabolized in viablesystems only. Major BPA metabolites produced by the skin were BPA mono-glucuronide and BPA monosulfate,accounting together for 73% and 27% of the dose, in pig and human, respectively. In conclusion,experiments with viable skin models unequivocally demonstrate that BPA is readily absorbed and metabolizedby the skin. The trans-dermal route is expected to contribute substantially to BPA exposure inhuman, when direct contact with BPA (free monomer) occurs.

Mots clés: skin; human; endocrine disrupter

Mots clés: bpa; dermal absorption; biotransformation

Domaines: Milieux et Changements globaux

Daniel Zalko, Carine Jacques, Hélène Duplan, Sandrine S. Bruel, Elisabeth Perdu. Viable skin efficiently absorbs and metabolizes bisphenol A. Chemosphere, 2011, 82 (3), pp.424-430. ⟨10.1016/j.chemosphere.2010.09.058⟩. ⟨hal-02650877⟩