Interaction between viologen-phosphorus dendrimers and α-synuclein

Katarzyna Milowska (1) , Justyna Grochowina (1) , Nadia Katir (2) , Abdelkrim El Kadib (3) , Jean Pierre Majoral (2) , Maria Bryszewska (1) , Teresa Gabryelak (1)

1 Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz
2 LCC - Laboratoire de chimie de coordination
3 ENSET - Ecole Normale Supérieure de l'Enseignement Technique [Rabat]

In this study the interaction between viologen-phosphorus dendrimers and α-synuclein (ASN) was examined. Polycationic viologen-phosphorus dendrimers (two positive charges per viologen unit) are novel compounds with relatively unknown properties. The influence of these viologen dendrimers on ASN was tested using fluorimetric and circular dichroism methods. ASN contains four tyrosine residues; therefore, the influence of dendrimers on protein molecular conformation by measuring the changes in the ASN fluorescence in the presence of dendrimers was evaluated. The interaction of dendrimers with free l-tyrosine was also monitored. Results show that viologen-phosphorus dendrimers interact with ASN; they quenched the fluorescence of ASN as well as free tyrosine by dynamic and static ways. However, the quenching was not accompanied by modifications in the ASN secondary structure.

Mots clés: Viologen-phosphorus dendrimers; α-Synuclein; Fluorescence quenching; Circular dichroism

Domaines: Chimie de coordination

Katarzyna Milowska, Justyna Grochowina, Nadia Katir, Abdelkrim El Kadib, Jean Pierre Majoral, et al.. Interaction between viologen-phosphorus dendrimers and α-synuclein. Journal of Luminescence, 2013, 134, pp.132-137. ⟨10.1016/j.jlumin.2012.08.060⟩. ⟨hal-02908261⟩