Formulation induces direct DNA UV-A photooxidation. Part II. Pro-oxidant effect of formulated Vitamin E via generation of singlet oxygen

Johanne Teychené (1) , Dalina Didacus-Prins (2) , Nadia Chouini-Lalanne (2, 3) , Christophe Dejugnat (2, 3) , Valérie Sartor (2, 3)

1 TBI - Toulouse Biotechnology Institute
2 IMRCP - Interactions moléculaires et réactivité chimique et photochimique
3 IMRCP - SMODD - IMRCP - Systèmes Moléculaires Organisés et Développement Durable

HIGHLIGHTS · Cetyltriethylammonium bromide (CTEAB) and Vitamin E self-assemble in TRIS/HCl buffer solutions into nanoemulsion composed of CTEAB micelles (that can incorporate Vitamin E) as well as larger Vitamin E droplets. · CTEAB-Vitamin E nanoemulsion interacts with plasmid DNA pBR322 and the gradual DNA compaction followed by gradual DNA decompaction for increasing CTEAB-Vitamin E amounts have been highlighted. · UVA irradiation induces photooxidation of DNA on the formulated CTEAB-Vitamin E-DNA lipoplex and underlines the pro-oxidant role of the Vitamin E in this self-assembled system.

Domaines: Chimie

Johanne Teychené, Dalina Didacus-Prins, Nadia Chouini-Lalanne, Christophe Dejugnat, Valérie Sartor. Formulation induces direct DNA UV-A photooxidation. Part II. Pro-oxidant effect of formulated Vitamin E via generation of singlet oxygen. Journal of Molecular Liquids, 2020, 316, pp.113818. ⟨10.1016/j.molliq.2020.113818⟩. ⟨hal-02916908⟩