Recording local field potential and neuronal activity with tetrodes in epileptic patients

Elodie Despouy (1, 2) , Jonathan Curot (1, 3) , Leila Reddy (1) , Lionel G Nowak (1) , Martin Deudon (1) , Jean-Christophe Sol (4, 5) , Jean-Albert Lotterie (4, 6) , Marie Denuelle (1, 3) , Ali Maziz (7) , Christian Bergaud (7) , Simon Thorpe (1) , Luc Valton (1, 3) , Emmanuel Barbeau (1)

1 CERCO - Centre de recherche cerveau et cognition
2 DIXI Medical
3 Pôle Neurosciences [CHU Toulouse]
4 ToNIC - Toulouse NeuroImaging Center
5 Service Neurochirurgie [CHU Toulouse]
6 Pôle imagerie médicale [CHU Toulouse]
7 LAAS - Laboratoire d'analyse et d'architecture des systèmes

Background: Recordings with tetrodes have proven to be more effective in isolating single neuron spiking activity than with single microwires. However, tetrodes have never been used in humans. We report on the characteristics, safety, compatibility with clinical intracranial recordings in epileptic patients, and performance, of a new type of hybrid electrode equipped with tetrodes. New Method: 240 standard clinical macroelectrodes and 102 hybrid electrodes were implanted in 28 patients. Hybrids (diameter 800 µm) are made of 6 or 9 macro-contacts and 2 or 3 tetrodes (diameter 70-80 µm). Results: No clinical complication or adverse event was associated with the hybrids. Impedance and noise of recordings were stable over time. The design enabled multiscale spatial analyses that revealed physiopathological events which were sometimes specific to one tetrode, but could not be recorded on the macro-contacts. After spike sorting, the single-unit yield was similar to other hybrid electrodes and was sometimes as high as > 10 neurons per tetrode. Comparison with Existing Method(s): This new hybrid electrode has a smaller diameter than other available hybrid electrodes. It provides novel spatial information due to the configuration of the tetrodes. The single-unit yield appears promising. Conclusions: This new hybrid electrode is safe, easy to use, and works satisfactorily for conducting multi-scale seizure and physiological analyses.

Domaines: Sciences du Vivant [q-bio]

Elodie Despouy, Jonathan Curot, Leila Reddy, Lionel G Nowak, Martin Deudon, et al.. Recording local field potential and neuronal activity with tetrodes in epileptic patients. Journal of Neuroscience Methods, 2020, 341, pp.108759. ⟨10.1016/j.jneumeth.2020.108759⟩. ⟨hal-03001304⟩